jak stat pathway inhibitors antibody sampler kit  (Cell Signaling Technology Inc)

Journal: Carcinogenesis

Article Title: PKK deletion in basal keratinocytes promotes tumorigenesis after chemical carcinogenesis

doi: 10.1093/carcin/bgx120

Figure Lengend Snippet: Deletion of PKK alters epidermal keratinocyte proliferation and signaling. (A) Immunofluoresence microscopy of C57BL6;129F10-PKKtm1(Tg(KRT14-cre/Esr1) back skin treated with vehicle or tamoxifen and stained against the basal keratinocyte differentiation marker keratin 14 and the proliferation marker Ki-67. Scale bar is 25 μm. (B) Ki-67 nuclear staining from three representative areas from WT and PKKEKO mouse skin was calculated. Standard error of means is shown. P ≤ 0.005. (C) and (D) Immunofluoresence microscopy of C57BL6;129F10-PKKtm1(Tg(KRT14-cre/Esr1) back skin treated with vehicle or tamoxifen stained against the basal keratinocyte differentiation marker keratin 5 or 14 and (c) TP63 or (e) phospho-p65. (D) and (F) TP63 and phospho-p65 staining from three representative areas from WT and PKKEKO mouse skin was calculated. Standard error of means is shown. P ≤ 0.01.

Article Snippet: Keratin 1/10 antibody (LH1, sc-53251), keratin 5 antibody (RCK-103, sc-32721), keratin 14 antibody (C-14, sc-17104), keratin 17 antibody (H-41, sc-366511), involucrin (H-20,sc-28557), phospho-p65 antibody (Cell Signaling #8214), p63 antibody (H-137, sc-8343), Ki-67 antibody (Cell Signaling, #12075), p65 antibody (Cell Signaling, #8242), cyclin D (M-20,sc-718) and staining were performed with a standard IF stain protocol.

Techniques: Microscopy, Staining, Marker

Journal: Carcinogenesis

Article Title: PKK deletion in basal keratinocytes promotes tumorigenesis after chemical carcinogenesis

doi: 10.1093/carcin/bgx120

Figure Lengend Snippet: Altered NF-κB and TP63 signaling in PKK-deleted keratinocytes. (A) Immunofluorescence microscopy of cultured keratinocytes from human SCCs that were treated with vehicle or 20 ng/ml of phorbol ester (TPA). p65 (red) and DAPI (blue). Scale bar is 25 μm. (b) Bar graphs show positive nuclear staining of cultured keratinocytes at 40× magnification. *P ≤ 0.05, **P ≤ 0.01. (C) Immunoblots against phosph-p65 in epidermis treated with TPA from C57BL6;129F10-PKKtm1(Tg(KRT14-cre/Esr1) wild-type or knockout. GAPDH is the loading control. All results were repeated at least two times. (D–F) Immunoblots of (D) IκBα and TP63 (E), p21 (F) and CDK4 of epidermis from control or PKKEKO mouse back skin. (G) Immunofluorescence microscopy of chemically induced tumors from wild-type or knockout mice. TP63 (red) and DAPI (blue). Scale bar is 50 μm. (H) Quantification of TP63 nuclear staining was counted at three representative areas of papillomas or SCCs at 40× magnification and is shown at right. *P ≤ 0.05, **P ≤ 0.01.

Article Snippet: Keratin 1/10 antibody (LH1, sc-53251), keratin 5 antibody (RCK-103, sc-32721), keratin 14 antibody (C-14, sc-17104), keratin 17 antibody (H-41, sc-366511), involucrin (H-20,sc-28557), phospho-p65 antibody (Cell Signaling #8214), p63 antibody (H-137, sc-8343), Ki-67 antibody (Cell Signaling, #12075), p65 antibody (Cell Signaling, #8242), cyclin D (M-20,sc-718) and staining were performed with a standard IF stain protocol.

Techniques: Immunofluorescence, Microscopy, Cell Culture, Staining, Western Blot, Knock-Out, Control